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Vector Laboratories fluorescent mounting with dapi
( A ) Immunofluorescence staining of LLC cells after 1 hour incubation with Carbenoxolone. 50K cells/well were seeded in a 24 well culture dish and incubated overnight. Cells were stained for nuclei with <t>DAPI</t> (blue) and either ICAM1 (Cy3-red) or HMGB1 (Alexa flour 488 -green). ( B ) Tumor sections of Carbenoxolone treated mice stained for ICAM1 and DAPI. n = 8. ( C ) Western blot analysis of ICAM1 (top) and HMGB1 (bottom) expression in LLC cells post 1 hour incubation with Carbenoxolone. Blots are cropped, full blots can be found in ( D ) Flow cytometry analysis of LLC cells incubated with for 1 <t>hour.</t> <t>Fluorescent</t> detection of ICAM1 was measured using Cy5. Duplicates of all samples provided the same effect.
Fluorescent Mounting With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescent mounting with dapi/product/Vector Laboratories
Average 98 stars, based on 1 article reviews
fluorescent mounting with dapi - by Bioz Stars, 2026-03
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Vector Laboratories agent vestashield mounting medium with dapi
( A ) Immunofluorescence staining of LLC cells after 1 hour incubation with Carbenoxolone. 50K cells/well were seeded in a 24 well culture dish and incubated overnight. Cells were stained for nuclei with <t>DAPI</t> (blue) and either ICAM1 (Cy3-red) or HMGB1 (Alexa flour 488 -green). ( B ) Tumor sections of Carbenoxolone treated mice stained for ICAM1 and DAPI. n = 8. ( C ) Western blot analysis of ICAM1 (top) and HMGB1 (bottom) expression in LLC cells post 1 hour incubation with Carbenoxolone. Blots are cropped, full blots can be found in ( D ) Flow cytometry analysis of LLC cells incubated with for 1 <t>hour.</t> <t>Fluorescent</t> detection of ICAM1 was measured using Cy5. Duplicates of all samples provided the same effect.
Agent Vestashield Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/agent vestashield mounting medium with dapi/product/Vector Laboratories
Average 86 stars, based on 1 article reviews
agent vestashield mounting medium with dapi - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier

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Vector Laboratories mounting medium vector laboratories h 1500 vestashield mounting medium
( A ) Immunofluorescence staining of LLC cells after 1 hour incubation with Carbenoxolone. 50K cells/well were seeded in a 24 well culture dish and incubated overnight. Cells were stained for nuclei with <t>DAPI</t> (blue) and either ICAM1 (Cy3-red) or HMGB1 (Alexa flour 488 -green). ( B ) Tumor sections of Carbenoxolone treated mice stained for ICAM1 and DAPI. n = 8. ( C ) Western blot analysis of ICAM1 (top) and HMGB1 (bottom) expression in LLC cells post 1 hour incubation with Carbenoxolone. Blots are cropped, full blots can be found in ( D ) Flow cytometry analysis of LLC cells incubated with for 1 <t>hour.</t> <t>Fluorescent</t> detection of ICAM1 was measured using Cy5. Duplicates of all samples provided the same effect.
Mounting Medium Vector Laboratories H 1500 Vestashield Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mounting medium vector laboratories h 1500 vestashield mounting medium/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
mounting medium vector laboratories h 1500 vestashield mounting medium - by Bioz Stars, 2026-03
96/100 stars
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86
Vector Laboratories vestashield mounting medium
( A ) Immunofluorescence staining of LLC cells after 1 hour incubation with Carbenoxolone. 50K cells/well were seeded in a 24 well culture dish and incubated overnight. Cells were stained for nuclei with <t>DAPI</t> (blue) and either ICAM1 (Cy3-red) or HMGB1 (Alexa flour 488 -green). ( B ) Tumor sections of Carbenoxolone treated mice stained for ICAM1 and DAPI. n = 8. ( C ) Western blot analysis of ICAM1 (top) and HMGB1 (bottom) expression in LLC cells post 1 hour incubation with Carbenoxolone. Blots are cropped, full blots can be found in ( D ) Flow cytometry analysis of LLC cells incubated with for 1 <t>hour.</t> <t>Fluorescent</t> detection of ICAM1 was measured using Cy5. Duplicates of all samples provided the same effect.
Vestashield Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vestashield mounting medium/product/Vector Laboratories
Average 86 stars, based on 1 article reviews
vestashield mounting medium - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier

86
Vector Laboratories dapi in vestashield
( A ) Immunofluorescence staining of LLC cells after 1 hour incubation with Carbenoxolone. 50K cells/well were seeded in a 24 well culture dish and incubated overnight. Cells were stained for nuclei with <t>DAPI</t> (blue) and either ICAM1 (Cy3-red) or HMGB1 (Alexa flour 488 -green). ( B ) Tumor sections of Carbenoxolone treated mice stained for ICAM1 and DAPI. n = 8. ( C ) Western blot analysis of ICAM1 (top) and HMGB1 (bottom) expression in LLC cells post 1 hour incubation with Carbenoxolone. Blots are cropped, full blots can be found in ( D ) Flow cytometry analysis of LLC cells incubated with for 1 <t>hour.</t> <t>Fluorescent</t> detection of ICAM1 was measured using Cy5. Duplicates of all samples provided the same effect.
Dapi In Vestashield, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi in vestashield/product/Vector Laboratories
Average 86 stars, based on 1 article reviews
dapi in vestashield - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier

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( A ) Immunofluorescence staining of LLC cells after 1 hour incubation with Carbenoxolone. 50K cells/well were seeded in a 24 well culture dish and incubated overnight. Cells were stained for nuclei with DAPI (blue) and either ICAM1 (Cy3-red) or HMGB1 (Alexa flour 488 -green). ( B ) Tumor sections of Carbenoxolone treated mice stained for ICAM1 and DAPI. n = 8. ( C ) Western blot analysis of ICAM1 (top) and HMGB1 (bottom) expression in LLC cells post 1 hour incubation with Carbenoxolone. Blots are cropped, full blots can be found in ( D ) Flow cytometry analysis of LLC cells incubated with for 1 hour. Fluorescent detection of ICAM1 was measured using Cy5. Duplicates of all samples provided the same effect.

Journal: Oncotarget

Article Title: High mobility group box 1 antagonist limits metastatic seeding in the lungs via reduction of cell–cell adhesion

doi: 10.18632/oncotarget.16188

Figure Lengend Snippet: ( A ) Immunofluorescence staining of LLC cells after 1 hour incubation with Carbenoxolone. 50K cells/well were seeded in a 24 well culture dish and incubated overnight. Cells were stained for nuclei with DAPI (blue) and either ICAM1 (Cy3-red) or HMGB1 (Alexa flour 488 -green). ( B ) Tumor sections of Carbenoxolone treated mice stained for ICAM1 and DAPI. n = 8. ( C ) Western blot analysis of ICAM1 (top) and HMGB1 (bottom) expression in LLC cells post 1 hour incubation with Carbenoxolone. Blots are cropped, full blots can be found in ( D ) Flow cytometry analysis of LLC cells incubated with for 1 hour. Fluorescent detection of ICAM1 was measured using Cy5. Duplicates of all samples provided the same effect.

Article Snippet: Slides were washed twice for 5 minutes and incubated with a secondary antibody labeled with Cy3 (mouse anti-rabbit, abcam) (1:500) in 3% normal goat serum for 1 hour and washed twice before fluorescent mounting with DAPI (VestaShield, Vector laboratories) applied on the slides, and samples were visualized using a fluorescent microscope (Olympus EX-73).

Techniques: Immunofluorescence, Staining, Incubation, Western Blot, Expressing, Flow Cytometry