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Image Search Results
Journal: Oncotarget
Article Title: High mobility group box 1 antagonist limits metastatic seeding in the lungs via reduction of cell–cell adhesion
doi: 10.18632/oncotarget.16188
Figure Lengend Snippet: ( A ) Immunofluorescence staining of LLC cells after 1 hour incubation with Carbenoxolone. 50K cells/well were seeded in a 24 well culture dish and incubated overnight. Cells were stained for nuclei with DAPI (blue) and either ICAM1 (Cy3-red) or HMGB1 (Alexa flour 488 -green). ( B ) Tumor sections of Carbenoxolone treated mice stained for ICAM1 and DAPI. n = 8. ( C ) Western blot analysis of ICAM1 (top) and HMGB1 (bottom) expression in LLC cells post 1 hour incubation with Carbenoxolone. Blots are cropped, full blots can be found in ( D ) Flow cytometry analysis of LLC cells incubated with for 1 hour. Fluorescent detection of ICAM1 was measured using Cy5. Duplicates of all samples provided the same effect.
Article Snippet: Slides were washed twice for 5 minutes and incubated with a secondary antibody labeled with Cy3 (mouse anti-rabbit, abcam) (1:500) in 3% normal goat serum for 1 hour and washed twice before
Techniques: Immunofluorescence, Staining, Incubation, Western Blot, Expressing, Flow Cytometry